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    • Still reflecting over the last weekend. I may gather my thoughts in an upcoming post. 4 days ago
    • My phone is fully charged, and we are preparing to head west in a few hours. 1 week ago
    • snow :( 1 week ago
    • No internet at home for two weeks, but now we are back on-line. This means less visits to my neighbor, who is my brother, who has internet. 1 week ago
    • I've started another blog to share different type of content that doesn't seem to fit atmybench. http://vitriscribble.blogspot.com/ 1 week ago
    • We tag ourselves. http://bit.ly/4noPee 2 weeks ago
    • Looking for an all black template in Wordpress, but no. Hello Blogspot? 2 weeks ago
    • We now enjoy keeping up with current events. http://bit.ly/3TxkGp 2 weeks ago
    • Correction: poster @ 3-5:30pm 3 weeks ago
    • I will be presenting a "work in progress" poster along with many other scientists tomorrow ~2pm in ADBF second floor. Come on by! 3 weeks ago
    • Today was the first time I have ever received the sacraments twice in one day. The extra rep was good. 1 month ago
    • I used Hydrochloric acid in stead of Acetic acid for my coomassie destain. No wonder it turned brown. 1 month ago
    • Shower, sushi and shiraz is very appetizing combo after a very long mountain bike ride. fyi 1 month ago
    • Poor wife, blood drawn and flu shot in one doctor's visit. She is taking one for the Laughey team today. 1 month ago
    • Considering Pr. 25:28 Like a city whose walls are broken down is a man who lacks self-control. 1 month ago
    • Considering Pr. 24:3 By wisdom a house is built up, and through understanding it is established. 1 month ago
    • Considering Pr. 23:29 Who has woe? Who has sorrow? Who has strife? Who has complaints? Who has needless bruises? Who has bloodshot eyes?... 1 month ago
    • The melding of current events. Youtube is great at this stuff. http://bit.ly/199FAA\ 1 month ago
    • My wife was exactly six months pregnant yesterday. 1 month ago

lab work snap shot: past, present, future

Posted by ljacobl on July 14, 2009

Past: I got a paper published, which is very nice to put in the  “past work” category. Writing a scientific manuscript for publication is painful and tedious and stressful and hair wrenching for me. I normally agonize over every sentence when I write; rearranging, retyping, and taking long pauses to formulate  thoughts and structure. Writing any thing takes me a long time. In fact these last 5 sentences have already taken quite a bit of time for me to pound out. Then, when I’m all done with writing what I want to bestow, I have to edit. I am a horrible speller and normally just guess at punctuation. I have to edit and edit and edit. And edit. I can’t tell you how may drafts of my manuscript I made.  It may actually be easier to figure out at what rate I produced drafts rather than the actual number. But, in the end, when it was all done, there was reward. The reward: not having to work on it anymore. It was a good experience though, and it’s nice to have my name published on something for future resumes. You can read the abstract and download my full article here. Have a good nap!

Present: For the first time in the history of my lab we are doing some in vivo cattle experiments with the parasites we study. This is to test whether the parasites that we modify are able to clinically infect the cattle (I have wrote about this before). This experiment has direct consequences on my project. If the cows get sick then it is a good sign that my my work will…work. It is eight days after inoculation and some of the calves are getting a fever ( a very good sign). In the picture are James and Ralph. They are very good at what they do, and care very much for their animals.

Paul's pictures 060

Future: I’m in the process of developing my own “mutant” parasite to inoculate into cattle. My parasite line will advance us one step closer to develop a dual vaccine system that will not only protect against the symptoms of the disease but also attack the tick vectors of the disease. Do you see GFP, BSD, and more importantly Bm86 expression from my transfected parasites in these contrast pictures? Well, it is my job to find out.

t140-1cellt140-1gfpedit

One Response to “lab work snap shot: past, present, future”

  1. lpappal said

    I’ve read this blog several times trying to understand in a little detail what you have accomplished. Way to go… You have done well.

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